Liangquan Zhu , Yafei Chang , Jing Li

School of Life Sciences and Biotechnology, Shanghai Jiaotong University, Shanghai, 200240
Author    Correspondence author
International Journal of Molecular Medical Science, 2020, Vol. 10, No. 3   
Received: 06 Mar., 2020    Accepted: 29 Mar., 2020    Published: 27 Apr., 2020

This article was first published in Genomics and Applied Biology in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

More and more evidence shows that the microbiome is closely related to liver diseases, but the taxa and the abundance of liver microbiome of patients with hepatitis B virus (HBV) -related hepatocellular carcinoma (HCC) are still not clear. In this study, we performed metaproteomics identification using mass spectrometry data from liver cancer tissue and corresponding adjacent tissue of 165 HCC patients. The results showed that in liver cancer tissue, the number of microorganisms, such as Chloroflexi, Gemmatimonadetes, Xanthomonadales, Legionellales and Oscillatoriales has increased significantly. Using COG (Clusters of Orthologous Groups) database to annotate identified proteins, we also found many function categories of microorganisms that are highly expressed in liver cancer tissue. Compared with the results of the proteomic identification of liver tissue from healthy people, we found that the expression level of Proteobacteria was reduced while the expression level of Firmicutes was increased in liver tissue of the HCC population. Our research could provide more inspiration for the association between dysbiosis of microbial flora in liver and the development of HCC.

Hepatitis B virus; Microbiome; Metaproteomics

(The advance publishing of the abstract of this manuscript does not mean final published, the end result whether or not published will depend on the comments of peer reviewers and decision of our editorial board.)