Yaming Zhao , Qiuxian Yang , Lingfeng Zuo , Zhan Yang , Yajun Yin , Jin Zhang

College of Biological and Chemical Science and Engineering, Jiaxing University, Jiaxing, 314001, China
Author    Correspondence author
International Journal of Molecular Medical Science, 2023, Vol. 13, No. 1   doi: 10.5376/ijmms.2023.13.0001
Received: 27 Feb., 2023    Accepted: 06 Mar., 2023    Published: 10 Mar., 2023

This article was first published in Genomics and Applied Biology in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Zhang Y.M., Yang Q.X., Zui L.F., Zhan Y., Yin Y.J., and Zhang J., 2023, Effects of saturated/unsaturated fatty acids on macrophage inflammatory status, International Journal of Molecular Medical Science, 13(1): 1-13 (doi: 10.5376/ijmms.2023.13.0001)

Studies have shown that fatty acids ingested into a body can affect the body's inflammatory status. In this study, eight kinds of fatty acids, which commonly presents in human daily diet, were added into bone marrow derived mouse microphages, respectively, then the mRNA abundance of the inflammation marker genes was analyzed to unveil the effects of fatty acids on macrophages inflammation. Results showed that saturated fatty acids, Palmitate acid (PA), Stearic acid (SA), Behenic acid (BA) and Lignoceric acid (LA), induced upregulation of TNFα, which indicated the macrophage became pro-inflammatory, and the effects of PA were highest. Unsaturated fatty acids, Palmitoleate acid (PO), Arachidonic acid (ARA), Eicosapentaenoic acid (EPA) and Docosahexaenoic acid (DHA), induced upregulation of IL10, which indicated the macrophage became anti-inflammatory, and the effects of DHA were the highest. Furthermore, added unsaturated fatty acids could reduce inflammatory states induced by PA, the effects of ARA were the most significant, followed by DHA. Western blot showed that the effects of PA, LA, ARA and DHA on macrophages inflammation were through NF-κB signaling pathway. In summary, saturated fatty acids PA, SA, BA and LA induce macrophages pro-inflammatory, while unsaturated fatty acid PO, ARA, EPA and DHA can alleviated macrophages inflammatory, with the best effects of ARA.

Fatty acid; Macrophage; NF-κB signaling pathway